体外研究
Tetraethylammonium (0.2-60 mM; 24-72 hours; C6 and 9L glioma cells) treatment inhibits the proliferation of C6 and 9L cells in a dose- and time-dependent manner.
Tetraethylammonium (40 mM; 24-72 hours; C6 and 9L glioma cells) treatment significantly increases apoptosis in cells.
Tetraethylammonium (40 mM; 12-48 hours; C6 and 9L glioma cells) treatment significantly elevates Bax/Bcl-2 protein ratio in a time-dependent manner.
The generation of intracellular ROS increased in C6 and 9L cells by the addition of 20 and 40 mM Tetraethylammonium.
Cell Proliferation Assay
Cell Line:
Rat C6 and 9L glioma cells
Concentration:
0.2 mM, 2 mM, 20 mM, 40 mM and 60 mM
Incubation Time:
24 hours, 48 hours and 72 hours
Result:
Inhibited the proliferation of C6 and 9L cells in a dose- and time-dependent manner.
Apoptosis Analysis
Cell Line:
Rat C6 and 9L glioma cells
Concentration:
40 mM
Incubation Time:
24 hours, 48 hours and 72 hours
Result:
Significantly increased apoptosis in cells.
Western Blot Analysis
Cell Line:
Rat C6 and 9L glioma cells
Concentration:
40 mM
Incubation Time:
12 hours, 24 hours, 48 hours
Result:
The expression of Bax was markedly increased, while that of Bcl-2 showed a decreasing trend 12, 24 and 48 h.