13860-38-3 | MFCD10687017 | N1-甲基-假尿苷

N1-甲基-假尿苷

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N1-甲基-假尿苷

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CAS No. 13860-38-3

分子式 C10H14N2O6

分子量 258.229

英文名称 1-methylpseudouridine

中文同义词 1-甲基-5-B-D-呋喃糖基-2,4(1H,3H)-嘧啶二酮;N1-甲基-假尿苷三磷酸溶液;1-甲基伪尿苷;N1-ME-假尿苷;N-甲基假尿苷;1-甲基假尿苷;5-((2S,3R,4S,5R)-3,4-二羟基-5-(羟甲基)四氢呋喃-2-基)-1-甲基嘧啶-2,4(1H,3H)-二酮;N1-甲基假尿苷固体

基本信息

产品名称

N1-甲基-假尿苷

中文同义词

1-甲基-5-B-D-呋喃糖基-2,4(1H,3H)-嘧啶二酮,N1-甲基-假尿苷三磷酸溶液,1-甲基伪尿苷,N1-ME-假尿苷,N-甲基假尿苷,1-甲基假尿苷,5-((2S,3R,4S,5R)-3,4-二羟基-5-(羟甲基)四氢呋喃-2-基)-1-甲基嘧啶-2,4(1H,3H)-二酮,N1-甲基假尿苷固体

英文名称

1-methylpseudouridine

英文同义词

1-methylpseudouridine;(-)-1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione;1-Methyl-5-(β-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione;1-Methyl-5-(β-D-ribofuranosyl)pyrimidine-2,4(1H,3H)-dione;Antibiotic U-50228;U-50228;N1-Methylpseudouridine;1-N-ME-PSEUDOURIDINE

CAS No.

13860-38-3

分子式

C10H14N2O6

分子量

258.229

EINECS号

MDL NO.

MFCD10687017

MOL

13860-38-3.mol

性质

密度

1.576±0.06 g/cm3(Predicted)

形态

固体

颜色

白色至米白色

InChIKey

UVBYMVOUBXYSFV-CAYFCZNJNA-N

储存条件

Store at -20°C

水溶解性

insoluble in Water;

酸度系数(pKa)

9.43±0.10(Predicted)

InChI

InChI=1/C10H14N2O6/c1-12-2-4(9(16)11-10(12)17)8-7(15)6(14)5(3-13)18-8/h2,5-8,13-15H,3H2,1H3,(H,11,16,17)/t5-,6-,7-,8+/s3

SMILES

[C@@H]1(O)[C@@H](CO)O[C@@H](C2C(=O)NC(=O)N(C)C=2)[C@@H]1O |&1:0,2,6,16,r|

溶解度

>20 mg/mL，乙醇溶液； >20.65 mg/mL，溶于 DMSO

用途

体内研究

N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. N1-methyl-pseudouridine (1-Methylpseudouridine) (20 μg; I.m. or i.d. routes for 21 days) and m5C/ N1-methyl-pseudouridine-modified mRNA respectively have a higher translational capacity than Ψ and m5C/Ψ-modified mRNA in vivo. Animal Model: 7-week-old Balb/c mice Dosage: 20 μg Administration: I.m. or i.d. routes for 21 days Result: had a higher translational capacity.

体外研究

Incorporation of N1-methyl-pseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA in HEK293T cells. Incorporation of N1-methyl-pseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the NN1-methyl-pseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate. In all the in vitro translation systems, incorporation of N1-methyl-pseudouridine in Luc and GFP mRNAs dramatically enhanced translation. The N1-methyl-pseudouridine-Luc mRNA is associated with heavier polysomes than Luc mRNA.

生物活性

N1-methyl-pseudouridine (1-Methylpseudouridine) 是一种甲基假尿苷，翻译性能优于 5 mC 和 5 mC/N1-methyl-pseudouridine。通过增加核糖体密度，mRNA 中的 N1-methyl-pseudouridine 以 eIF2α 依赖性和独立机制增强翻译。

安全信息